Technical Data
P4072-03D
Phosphodiesterase 4A, phosphorylated (Phosphodiesterase 4A cAMP-specific, Phosphodiesterase-4A, PDE4A, cAMP Specific 3’ 5’ Cyclic Phosphodiesterase 4A, cAMP Specific Phosphodiesterase, Cyclic AMP Phosphodiesterase PDE4A11, Cyclic AMP Specific Phosphodiest
Description:
This antibody is ideal for studying phosphorylation dependent signal transduction research of PDE4 activation.

Cellular Localization: Cell Membrane

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to PKA phosphorylation site on PDE4A long form enzymes. The peptide was synthesized with phosphorylated serine.
Purity:
Purified by immunoaffinity chromatography.
Form
0.02% Thimerosal / Merthiolate
Specificity:
This antibody detects all known long-from variants of PDE4A, including PDE4A4, PDE4A5, PDE4A8, PDE4Ax when phosphorylated at the PKA phosphorylation site. It will not crossreact with PDE4A phosphorylated at the ERK phosphorylation site. It does not crossreact with PDE4B, PDE4D or any other member of the PDE family. Species Crossreactivity: Human (weakly), Mouse, Rat and Rabbit.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
This product has been used in:Zhu B et al. Activation and induction of cyclic AMP phosphodiesterase (PDE4) in rat pulmonary microvascular endothelial cells. Biochem Pharmacol 68:479-91 (2004). Zhang HT et al. Inhibition of cyclic AMP phosphodiesterase (PDE4) reverses memory deficits associated with NMDA receptor antagonism. Neuropsychopharmacology 23:198-204 (2000). Yarwood SJ et al. The RACK1 signaling scaffold protein selectively interacts with the cAMP-specific phosphodiesterase PDE4D5 isoform. J Biol Chem 274:14909-17 (1999). Beavo JA Cyclic nucleotide phosphodiesterases: functional implications of multiple isoforms. Physiol Rev 75:725-48 (1995).