Technical Data
P4072-03H
Phosphodiesterase 4B (PDE4B, PDEIVB, cAMP-specific 3',5'-cyclic Phosphodiesterase 4B, DPDE4, PDE32)
Description:
PDE4B is a member of the type IV, cyclic AMP (cAMP)-specific, cyclic nucleotide phosphodiesterase (PDE) family. Enzymes of the cAMP-dependent phosphodiesterase type 4 (PDE4) family are important in hydrolyzing cAMP produced by G-protein coupled receptor (GPCR) stimulated adenylyl cyclases. Four genes (PDE4A, B, C and D) make up the PDE4 family. cAMP-dependent phosphodiesterase type B (PDE4B) family consists of four known splice variants, PDE4B1, B2, B3 and a novel 66kD PDE4B4 protein. One or more subtype variants of PDE4B are ubiquitously present in all mammalian cells. In CNS, all four PDE4B subtype variants are expressed in varying ratios and their activity is regulated in tandem with GPCRs stimulation. Some PDE4B variants are also substrate for PKA-dependent phosphorylation. Peripheral tissues exhibit differential expression of PDE4B1, B2 and B4 variants; the PDE4B4 (66kD protein) is the major PDE4B variant expressed in lungs, testis and heart. In testis PDE4B enzymes are expressed in somatic cells (Sertoli and Leydig cells) while PDE4A is localized in germ cells.

Applications:
Suitable for use in Immunofluorescence, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:200
Western Blot: 1:1500 detects a band at 107kD (PDE4B1), 78kD (PDE4B2), 100kD (PDE4B3) and 66kD (PDE4B4).
Immunoprecipitation: 1:350
Immunocytochemistry: 1:300
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to the N-terminal of human Phosphodiesterase 4B.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, 0.02% thimerosal, 20% glycerol.
Specificity:
Recognizes human Phosphodiesterase 4B. Reacts with all PDE4B proteins including PDE4B1, PDE4B2, PDE4B3 and a 66kD PDE4B variant. Does not crossreact with PDE4A, PDE4C or PDE4D. Species Crossreactivity: mouse, rat, chicken and primate
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Farooqui SM et al. Surgically induced cryptorchidism-related degenerative changes in spermatogonia are associated with loss of cyclic adenosine monophosphate-dependent phosphodiesterases type 4 in abdominal testes of rats. Biol Reprod 64:1583-9 (2001). Huston E et al. Molecular cloning and transient expression in COS7 cells of a novel human PDE4B cAMP-specific phosphodiesterase, HSPDE4B3. Biochem J 328 ( Pt 2):549-58 (1997).