Technical Data
P4072-05B
Phosphodiesterase 5A (Phosphodiesterase 5A cGMP-specific, PDE5A, PDE-5A, PDE5A1, cGMP Binding cGMP Specific 3' 5' Cyclic Nucleotide Phosphodiesterase, cGMP-binding cGMP-specific 3 5-cyclic Nucleotide Phosphodiesterase, cGMP Binding cGMP Specific Phospho
Description:
The cyclic monophosphate nucleotides are found ubiquitously in mammalian cells and act as second messenger transducers to effect the intracellular actions of a variety of G-protein coupled receptors (GPCRs) for hormones, cytokines, and neurotransmitters. Cyclic nucleotides are important intracellular second messengers which play important role in a variety of signal transduction process. The cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodieterases. One of the many phosphodiesterases that compartmentalized and hydrolyze cGMP in various tissues is phosphodiesterase type 5A. The cGMP is involved in nitric oxide signaling as well as cell signaling associated with natriuretic peptides and gulanylins. Some of the intracellular biding cites for the cGMP include cyclic nucleotide gated ion channels, cGMP-dependent protein kinases, and cyclic GMP biding phosphodiesterases (cGB-PDEs). The cGB-PDEs include PDE2, PDE3, PDE5, PDE5 and PDE10; the members of these families contain various structural and functional motifs that are conserved. Most of these proteins contain dimeric subunits that contain a highly conserved cGMP binding site and a phosphodiesterase catalytic site.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500
Immunoprecipitation: 1:250
Optimal dilutions to be determined by the researcher.

Positive Control:
Rat lung homogenate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic cyclic peptide corresponding to rat Phosphodiesterase 5A Sequence homology: common to all PDE5A variants. Cellular Localization: Located mostly to soluble cellular fractions, and sometimes to particulate cellular fractions.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid, 0.02% sodium azide.
Specificity:
Recognizes rat Phosphodiesterase 5A. Detects 99kD and 89kD PDE5A proteins in various tissues. Species Crossreactivity: human and mouse .
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rybalkin SD et al. J Biol Chem 277:3310-7 (2002). 2.Murthy KS Biochem J 360:199-208 (2001). McAllister-Lucas LM et al. The structure of a bovine lung cGMP-binding, cGMP-specific phosphodiesterase deduced from a cDNA clone. J Biol Chem 268:22863-73 (1993). Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991).