Technical Data
Phosphodiesterase 6 alpha (Phosphodiesterase 6A alpha Subunit, Phosphodiesterase 6A cGMP-specific Rod alpha, PDE6 alpha, PDE6A, CGPR-A, GMP-PDE alpha, PDE V-B1, PDEA, Retinal Rod Photoreceptor cGMP Phosphodiesterase alpha, Rod cGMP-specific 3' 5'-cyclic P
The second messengers cAMP and cGMP are key regulatory molecules that are involved in a wide variety of signal transduction pathways, such as insulin secretion, platelet aggregation, smooth muscle relaxation, olfaction, and vision. Levels of cAMP and cGMP are regulated by their rate of synthesis by nucleotide cyclases and by their rate of hydrolysis by cyclic nucleotide phosphodiesterases (PDEs). PDEs form a superfamily of enzymes that catalyze the conversion of 3-prime, 5-prime-cyclic nucleotides to the corresponding nucleoside 5-prime-monophosphates. While mammalian PDEs are divided into major families based on their substrate specificities, kinetic properties, allosteric regulators, inhibitor sensitivities, and amino acid sequences, each family and even members within a family display distinct tissue, cell, and subcellular expression patters. This suggests that individual PDE family members are involved in discrete signal transduction pathways. PDE6 is the effector enzyme in the G protein-mediated signal transduction cascade in the visual system. There are five different subunits consisting of rod and cone specific catalytic subunits: alpha? (Cone), alpha (Rod), and beta (Rod), the inhibitory subunit gamma, and subunit delta of unknown function (which likely interacts with many other proteins besides the PDE6 family). The catalytic core of the PDE6 system is comprised of alpha?/alpha? homodimers in the cone and alpha/beta heterodimers in the rod. The C-terminus of both the catalytic and inhibitory subunits is modified by methylation, myristyolation and prenylation which have been shown to be critical for proper complex assembly and membrane association.

Cellular Localization: Plasma membrane

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide: NPQFAKEYFDRKLR, corresponding to amino acids 16-29 of Human PDE6 alpha. This sequence is completely conserved between human, chicken and frog.
As reported
Supplied as a liquid in PBS, pH 7.2, 1mg/ml BSA, 0.05% sodium azide.
Species Crossreactivity: Sheep.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
He F et al. Multiple zinc binding sites in retinal rod cGMP phosphodiesterase, PDE6alpha beta. J Biol Chem 275:20572-7 (2000). Granovsky AE & Artemyev NO Identification of the gamma subunit-interacting residues on photoreceptor cGMP phosphodiesterase, PDE6alpha '. J Biol Chem 275:41258-62 (2000).