Technical Data
P4072-10B
Phosphodiesterase 10A (PDE10A, cAMP and cAMP-inhibited cGMP 3'5'-cyclic Phosphodiesterase 10A, FLJ11894, HSPDE10A, Phosphodiesterase 10A1)
Description:
One of the many phosphodiesterases that compartmentalize and hydrolyze cAMP and cGMP into AMP and GMP is PDE10. Only one member of the cAMP and cGMP-specific PDE10 family has been cloned and characterized. The PDE10A gene is expressed in at least two variant forms (PDE10A1, PDE10A2) generated by RNA splicing and/or the use of alternate initiation sites. PDE10A is widely expressed in the putamen and caudate nucleus in the brain, thyroid and testis. The expression of PDE10A is also abundant in Heart.

Applications:
Suitable for use in Western Blot, Immunoprecipitation, Immunofluorescence and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation: 1:250
Western Blot: 1:1500 detects a band at ~90-93kD in rat brain and rat pancreas samples
Immunohistochemistry: paraffin sections
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to a common sequence near aa250-325, downstream from the N terminal region of human Phosphodiesterase 10A. Cellular Localization: Located mostly to soluble cellular fractions.
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in stabilization buffer, 0.02% thimerosal.
Specificity:
Recognizes human Phosphodiesterase 10A. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Kotera J et al. Subcellular localization of cyclic nucleotide phosphodiesterase type 10A variants, and alteration of the localization by cAMP-dependent protein kinase-dependent phosphorylation. J Biol Chem 279:4366-75 (2004). 2. Fujishige K et al. Striatum- and testis-specific phosphodiesterase PDE10A isolation and characterization of a rat PDE10A. Eur J Biochem 266:1118-27 (1999).