Technical Data
P4072C1
Phosphodiesterase 1C (Phosphodiesterase 1C Calmodulin Dependent 70kDa, Phosphodiesterase 1C Calmodulin-dependent, PDE1C, Calcium/calmodulin-dependent 3'5'-cyclic Nucleotide Phosphodiesterase 1C, Cam-PDE-1C, hCAM3, hCAM-3, HSPDE1C1A, Human 3'5' Cyclic Nucl
Description:
PDE1C is a phosphodiesterase activated by the binding of calmodulin in the presence of calcium. Cyclic nucleotides are hydrolyzed and compartmentalized by a family of enzymes called phosphodiesterases. In mammals at least 11 different families of PDEs can be discriminated (PDE1-11) based on their kinetic properties and inhibition to various pharmacological agents. Three different PDE1 genes have been identified (PDE1A, PDE1B, PDE1C). The enzymes of the PDE1A and PDE1B have higher affinity for cGMP than for cAMP, where as, PDE1C has high affinity for both cAMP and cGMP. Calcium-Calmodulin stimulates the activity of each of these enzymes several-fold. The PDE1C gene codes a family of proteins (variants) that differ from each other at amino and carboxy termini of the protein. The tissue distribution of PDE1C is different from other PDE1 gene family members.

Applications:
Suitable for use in Immunohistochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Frozen/paraffin): 1:10-1:500
Immunoprecipitation: 1:10-1:500
Western Blot: 1:250-1:500
Note: In Immunoprecipitation and Western Blot, this antibody may exhibit mulitple bands in the 70-87kD range which are believed to be various PDE1C variants.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.75mg/ml
Immunogen:
Synthetic peptide (N terminal).
Purity:
Purified by immunoaffinity chromatography
Form
Supplied as a liquid, 0.02% thimerosal
Specificity:
Recognizes human Phosphodiesterase 1C. Does not crossreact with PDE1A, PDE1B or other PDEs. Species Crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Loughney K et al. Isolation and characterization of cDNAs corresponding to two human calcium, calmodulin-regulated, 3',5'-cyclic nucleotide phosphodiesterases. J Biol Chem 271:796-806 (1996). 2. Yan C et al. Molecular cloning and characterization of a calmodulin-dependent phosphodiesterase enriched in olfactory sensory neurons. Proc Natl Acad Sci U S A 92:9677-81 (1995). 3. Farooqui SM et al. Antibodies against synthetic peptides predicted from the nucleotide sequence of D2 receptor recognize native dopamine receptor protein in rat striatum. J Neurochem 57:1363-9 (1991).