Technical Data
P4073-52A
Phospholamban
Description:
Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and most Functional applications. Other applications not tested.

Recommended Dilutions:
Immunocytochemistry: 20ug/ml
Immunohistochemistry: Paraffin sections
Western Blot: 2ug/ml Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB).
Functional Applications: Increases the calcium uptake in ventricular SR vesicles.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing aliquot and store at -20C. Aliquots are stable for 12 months after receipt at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6D657Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceCanineMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa 2-25 of canine PLB (phospholamban), D2KVQYLTRSAIRRASTIEMPQQAR25.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, 0.05% sodium azide.
Specificity:
Recognizes canine Phospholamban. Recognizes an epitope between aa9-17 of canine PLB. Species Crossreactivity: human, mouse, rat, rabbit, chicken, sheep, porcine, guinea pig
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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