Description:
Applications:
Suitable for use in Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot and most Functional applications. Other applications not tested.
Recommended Dilutions:
Immunocytochemistry: 20ug/ml
Immunohistochemistry: Paraffin sections
Western Blot: 2ug/ml Detects bands of ~25kD (non-reducing conditions; representing the pentameric form of PLB) and ~5kD (reducing conditions; representing the monomeric form of PLB).
Functional Applications: Increases the calcium uptake in ventricular SR vesicles.
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG2a | 6D657 | Affinity Purified |
|
| Concentration: |
| Not Determined |
| Immunogen: |
| Synthetic peptide corresponding to aa 2-25 of canine PLB (phospholamban), D2KVQYLTRSAIRRASTIEMPQQAR25. |
| Purity: |
| Purified by Protein A affinity chromatography. |
| Form |
| Supplied as a liquid in PBS, 0.05% sodium azide. |
| Specificity: |
| Recognizes canine Phospholamban. Recognizes an epitope between aa9-17 of canine PLB. Species Crossreactivity: human, mouse, rat, rabbit, chicken, sheep, porcine, guinea pig |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. J.S.K. Sham, et al.; Am. J. Physiol. 261, H1344 (1991) 2. F.N. Briggs, et al.; J. Biol. Chem. 267, 26056 (1992) 3. L.R. Jones et al; J. Biol. Chem. 268, 11486 (1993) 4. T. Cantilina, et al.; J. Biol. Chem. 268, 17018 (1993) 5. A.O. Jorgensen, et al.; J. Cell Biol. 120, 969 (1993) 6. H.A. Rockman, et al.; PNAS 91, 2694 (1994) 7. B. O’Rourke, et al.; Circ. Res. 84, 562 (1999) 8. S.U. Trost, et al.; Diabetes 51, 1166 (2002) 9. L.S. Song, et al.; Circ. Res. 90, 174 (2002) 10. M.T. Jiang, et al.; Circ. Res. 91, 1015 (2002) 11. F. del Monte, et al.; Circulation 105, 904 (2002) 12. A.N. Carr, et al.; Mol. Cell. Biol. 22, 4124 (2002) 13. S. Huke, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H958 (2002) 14. K.M. Choi, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H1398 (2002) 15. B. Chaudhri, et al.; Am. J. Physiol. Heart Circ. Physiol. 283, H2450 (2002) 16. Y. Ji, et al.; J. Biol. Chem. 278, 25063 (2003) 17. K.Y. Xu, et al.; J. Biol. Chem. 278, 41798 (2003) 18. Y.K. Kim, et al.; J. Biol. Chem. 278, 47622 (2003)
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