Technical Data
PLA2G4A, ID (Cytosolic Phospholipase A2, cPLA2, Phospholipase A2 Group IVA, Phospholipase A2, Phosphatidylcholine 2-acylhydrolase, Lysophospholipase, PLA2G4, CPLA2)
PLA2G4A is a member of the cytosolic phospholipase A2 group IV family. The enzyme catalyzes the hydrolysis of membrane phospholipids to release arachidonic acid which is subsequently metabolized into eicosanoids. Eicosanoids, including prostaglandins and leukotrienes, are lipid-based cellular hormones that regulate hemodynamics, inflammatory responses, and other intracellular pathways. The hydrolysis reaction also produces lysophospholipids that are converted into platelet-activating factor. The enzyme is activated by increased intracellular Ca(2+) levels and phosphorylation, resulting in its translocation from the cytosol and nucleus to perinuclear membrane vesicles.

Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry, ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry (FFPE): 1:10-1:50
Immunofluorescence: 1:10-1:50
Flow Cytometry: 1:10-1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
200ul-20CBlue IceHumanRabbit
As reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 513-541 in the central region of human PLA2G4A, UniProt Accession #NP_077734.1; P47712. Species sequence homology: Chicken, mouse, rat, zebrafish
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human PLA2G4A.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Sharp,J.D.,, J. Biol. Chem. 266(23), 14850-14853 (1991). 2. Clark,J.D.,, Cell 65(6), 1043-1051 (1991).