Technical Data
Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation.

Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Can also be used for conjugation to label including enzymes, fluorochromes, biotin and agarose. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-10,000
Western Blot: 1:1000-1:2000
Immunoprecipitation: 2-5ug/IP reaction
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
Affinity purified native tyrosine phosphorylated proteins
Purified by affinity chromatography.
Supplied as a liquid in PBS, pH 7.4, 0.09% sodium azide.
Recognizes human Phosphotyrosine. Specific to phosphotyrosine containing proteins. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins. Species Crossreactivity: tyrosine-phosphorylated proteins from any species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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