Technical Data
P4075-32
Phosphotyrosine
Description:
Phosphorylation of tyrosine residues is associated with many growth factor receptors and oncogene protein kinases, and is an important signal for cellular activation, proliferation and differentiation.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation and Immunohistochemistry. Can also be used for conjugation to label including enzymes, fluorochromes, biotin and agarose. Other applications not tested.

Recommended Dilutions:
ELISA: 1:5000-10,000
Western Blot: 1:1000-1:2000
Immunoprecipitation: 2-5ug/IP reaction
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Affinity purified native tyrosine phosphorylated proteins
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Phosphotyrosine. Specific to phosphotyrosine containing proteins. Minimal to no crossreactivity with phosphothreonine or phosphoserine containing proteins. Species Crossreactivity: tyrosine-phosphorylated proteins from any species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hunter, T., Cell 50: 823 (1987). 2. Sorkin, A. & Carpenter, G., Science 261: 612-615 (1993). 3. Soler, C., et al., J. Biol. Chem. 269: 12,320-12,324 (1994). 4. Kiener, P.A., et al., J. Immunology 155: 4917-4925 (1995). 5. Khurana, S., et al., J. Biol. Chem. 271: 9919-9927 (1996). 6. Khurana, S., et al., J. Biol. Chem. 272: 30,115-30,121 (1997). 7. Palumbo, G.A., et al., Cancer Research 57: 2434-2439 (1997). 8. Khurana, S., et al., J. Biol. Chem. 271: 9919-9927 (1996). 9. Poppleton, H., et al., J. Biol. Chem. 271: 6947-6951 (1996). 10. Chen, Z., et al., J. Biol. Chem. 270: 27,525-27,530 (1995). 11. Chen, J.K., et al., J. Biol. Chem. 274: 4764-4769 (1999). 12. Chen, J.K., et al., J.Biol. Chem. 273: 29,254-29,261(1998). 13. Yipt, J.W., et al., J. Biol. Chem. 272: 18,473-18,480 (1997). 14. Maruyama, T., et al., Endocrinology 140: 5982-5990 (1999).