Technical Data
P4079-01D
(6-4) Photoproducts
Description:
This antioody is intended for the detection of DNA damage as a result of UV irradiation.

Hybridoma:
Mouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) spleen cells

Applications:
Suitable for use in ELISA and DNA Blotting. Other applications have not been tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1KTM50Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.5mg/ml
Immunogen:
UV-irradiated salmon sperm DNA
Purity:
Purified
Form
Supplied as a liquid in PBS containing 0.1% sodium azide and protein stabilizer
Specificity:
Recognizes (6-4) photoproducts of thymine dimers that are produced by UV-irradiation of DNA. Does not react with thymine dimers. Binds (6-4) photoproducts in both double and single stranded DNA, although the affinity is less for single stranded DNA. May react with normal human DNA under various conditions.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Eller, M.S. et al. (1997). Enhancement of DNA repair in human skin cells by thymidine dinucleotides: Evidence for a p53-mediated mammalian SOS response. Proc. Natl. Acad. Sci. USA 94: 12627-12632.