Description:
This antioody is intended for the detection of DNA damage as a result of UV irradiation.
Hybridoma:
Mouse myeloma (P3/X63-Ag8) x immunized mouse (Balb/c) spleen cells
Applications:
Suitable for use in ELISA and DNA Blotting. Other applications have not been tested.
Recommended Dilution:
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|
| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | KTM50 | Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~0.5mg/ml |
| Immunogen: |
| UV-irradiated salmon sperm DNA |
| Purity: |
| Purified |
| Form |
| Supplied as a liquid in PBS containing 0.1% sodium azide and protein stabilizer |
| Specificity: |
| Recognizes (6-4) photoproducts of thymine dimers that are produced by UV-irradiation of DNA. Does not react with thymine dimers. Binds (6-4) photoproducts in both double and single stranded DNA, although the affinity is less for single stranded DNA. May react with normal human DNA under various conditions. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Eller, M.S. et al. (1997). Enhancement of DNA repair in human skin cells by thymidine dinucleotides: Evidence for a p53-mediated mammalian SOS response. Proc. Natl. Acad. Sci. USA 94: 12627-12632.
|
|
