Technical Data
PIAS2 (Protein inhibitor of activated STAT, 2 Androgen, receptor-interacting protein 3, ARIP3, DAB2-interacting protein, DIP, E3 SUMO-protein ligase PIAS2, MGC102682, miz, Miz1, MIZ1, Msx-interacting zinc finger protein, PIAS-NY protein, PIASX, PIASX-AL
PIAS2 (Protein inhibitor of activated STAT2; also DIP, MIZ1 and PIASx- ) is a 45-70kD member of the PIAS family of molecules. In primary cells and tissues it is expressed in T and NK cells, and in testis where it likely regulates spermatogenesis. However, it is widely expressed in cultured cell lines. PIAS2 is known to inhibit STAT4-mediated gene activation, and to interact with inactive ELK-1, promoting its activation via HDAC2 dissociation and desumoylation. Human PIAS2 is 621 amino acids (aa) in length. It contains a SAP domain (aa11-45), a zinc-finger region (aa331-408), a SUMO-binding motif (aa467-472) and an NLS (aa484-492). There are multiple splice variants. PIAS2x- /ARIP3 shows a 22 aa substitution for aa 551-621, while PIAS-NY shows a 12 aa substitution for aa 1-8, followed by a truncation after Gly401. Two others show a four aa substitution for aa 504-621, and an alternate start site at Met10. Over aa 1-550, human PIAS2 shares 98% aa identity with mouse PIAS2.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute by adding sterile 40-50% glycerol, PBS, 0.02% sodium azide. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanSheep
Purified, E. coli-derived recombinant human PIAS2 aa 1-550; Accession# O75928.
Purified by immunoaffinity chromatography.
Supplied as a lyophilized powder in PBS, 5% trehalose. Reconstitute with 100ul sterile 40-50% glycerol, PBS, 0.02% sodium azide.
Detects endogenous human PIAS2 in Western blot with an approximate molecular weight of 63-70kD.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.