Technical Data
P4085-11B
PIAS2, CT (E3 SUMO-protein Ligase PIAS2, Protein Inhibitor of Activated STAT2, Protein Inhibitor of Activated STAT x, Msx-interacting Zinc Finger Protein, Miz1, DAB2-interacting Protein, DIP, Androgen Receptor-interacting Protein 3, ARIP3, PIAS-NY Protein
Description:
PIASX functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role as a transcriptional coregulator in various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. The effects of this transcriptional coregulation, transactivation or silencing may vary depending upon the biological context and the PIAS2 isoform studied. However, it seems to be mostly involved in gene silencing. PIASX binds to sumoylated ELK1 and enhances its transcriptional activity by preventing recruitment of HDAC2 by ELK1, thus reversing SUMO-mediated repression of ELK1 transactivation activity. Isoform PIAS2-beta, but not isoform PIAS2-alpha, promotes MDM2 sumoylation. Isoform PIAS2-alpha promotes PARK7 sumoylation. Isoform PIAS2-beta promotes NCOA2 sumoylation more efficiently than isoform PIAS2-alpha.

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the C-terminal region of human PIASx1/2 (KLH).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human PIAS2.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Arora, T., et al., J. Biol. Chem. 278(24):21327-21330 (2003). 2. Wu, S., et al., Oncogene 22(3):351-360 (2003). 3. Liu, B., et al., Proc. Natl. Acad. Sci. USA 95(18):10626-10631 (1998). 4. Wu, L., et al., Mech. Dev. 65 (1-2), 3-17 (1997).