Technical Data
P4086
PIAS1, CT (Protein Inhibitors of Activated STAT)
Description:
PIAS1 (protein inhibitor of activated STAT) proteins blocks the DNA binding activity of Stat1 and inhibits Stat1-mediated gene activation in response to cytokine stimulation (1). PIAS1 also plays a crucial role as a transcriptional coregulator in the p53 pathway and the steroid hormone signaling pathway and functions as an E3 type small ubiquitin-like modifier (SUMO) ligas, stabilizing the interaction between UBEZ1 and the substrate, and as a SUMO-tethering factor. It is expressed in numerous tissues with highest level in testes.

Applications:
Suitable for use in ELISA, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:5000-1:50,000
Immunohistochemistry: 1:100-1:250
Immuocytochemistry: 1: 100-1:250
Immunoprecipitation: 1:40
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9E445Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
A synthetic peptide corresponding to residues on the C-terminus of human PIAS1.
Purity:
Supernatant
Form
Supplied as a liquid in 50 mM Tris-Glycine, pH 7.4, 0.15 M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol.
Specificity:
Recognizes human PIAS1, CT at ~78kD. Species crossreactivity: mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu B, et al. Proc Natl Acad Sci 95(18):10626-31, 1998