Technical Data
PIAS1, NT (E3 SUMO-protein Ligase PIAS1, Protein Inhibitor of Activated STAT Protein 1, Gu Binding Protein, GBP, RNA Helicase II Binding Protein, DEAD/H Box-binding Protein 1, DDXBP1)
PIAS1 functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. This protein plays a crucial role in transcriptional coregulation of various cellular pathways, including the STAT pathway, the p53 pathway and the steroid hormone signaling pathway. It functions in testis as a nuclear receptor transcriptional coregulator and may have a role in androgen receptor initiation and maintenance of spermatogenesis. The effects of transcriptional coregulation, transactivation or silencing, may vary depending upon the biological context.

Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
200ul-20CBlue IceHumanRabbit
As reported
KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 100-130 in the N-terminal region of human PIAS1, UniProt Accession #O75925.
Purified by ammonium sulfate precipitation.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes human PIAS1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Miyauchi, Y., et al., J. Biol. Chem. 277(51):50131-50136 (2002). 2. Nishida, T., et al., J. Biol. Chem. 277(44):41311-41317 (2002). 3. Tan, J.A., et al., J. Biol. Chem. 277(19):16993-17001 (2002). 4. Megidish, T., et al., J. Biol. Chem. 277(10):8255-8259 (2002). 5. Liu, B., et al., J. Biol. Chem. 276(39):36624-36631 (2001).