Technical Data
Media
Pichia Amino Acid Mixture (Powder)
P4100
Molecular Biology Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
L-Glutamic Acid5g
L-Isoleucine5g
L-Leucine5g
L-Lysine5g
L-Methionine5g
Total:25g/L
A mixture of essential amino acids for use in Pichia pastoris growth and expression media.


Directions per Liter: Dissolve 25g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to1L. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi).

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: White, homogeneous, free flowing powder

Solubility: Colorless, clear, complete

pH: As Reported


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.