Technical Data
P4184-73
PI3 Kinase Class III
Description:
Three distinct types of phosphoinositide 3-kinases (PI3K) have been characterized. Unlike other PI3Ks, PI3K class III catalyzes the phosphorylation of phosphatidylinositol at the D3 position, producing phosphatidylinositol-3-phosphate (PI(3)P) (1). PI3K class III is the mammalian homolog of Vps34, first identified in yeast. PI3K class III interacts with the regular subunit p150, the mammalian homolog of Vps15, which regulates cellular membrane association through myristoylation (2,3). PI(3)P recruits several proteins with FYVE or PX domains to membranes regulating vesicular transport and protein sorting (4). Moreover, PI3K class III has been shown to regulate autophagy, trimeric G-protein signaling, and the mTOR nutrient-sensing pathway (5). Specificity/Sensitivity: PI3 Kinase Class III Antibody detects endogenous levels of total PI3K class III protein. This antibody may cross-react with an unidentified protein at ~55kD.

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
mmunoprecipitation: 1:50
Optimal dilutions to be determined by the researcher.

Storage and Stability:
For long-term storage, aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Immunogen:
Synthetic peptide (KLH-coupled) corresponding to human PI3K class III.
Purity:
Purified peptide affinity chromatography.
Form
Supplied as a liquid in10 mM sodium HEPES (pH 7.5), 150 mM NaCl, 100ug/ml BSA and 50% glycerol.
Specificity:
Detects endogenous levels of total PI3K class III protein. This antibody may cross-react with an unidentified protein at ~55kD. Species Crossreactivity: Human, mouse, rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Odorizzi, G. et al. (2000) Trends Biochem Sci 25, 229–35.
(2) Panaretou, C. et al. (1997) J Biol Chem 272, 2477–85.
(3) Kihara, A. et al. (2001) J Cell Biol 152, 519–30.
(4) Corvera, S. (2001) Traffic 2, 859–66.
(5) Yan, Y. and Backer, J.M. (2007) Biochem Soc Trans 35, 239–41.