Technical Data
P4214-31
Plectin
Description:
Plectin, a 500kD intermediate filament binding protein, is localized to the cytoplasm and acts to both stabilize and give strength to cells via cross-linking (1). Plectin cross-links intermediate filaments to microtubules and microfilaments, and anchors intermediate filaments to the plasma and nuclear membranes (2,3). Plectin binds many proteins, including actin, vimentin, and Lamin B. Phosphorylation by PKA or PKC results in decreased binding to Lamin B, and phosphorylation by PKA enhances the plectin-vimentin interactions (4).

Plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemidesmosomes, Z-line structures and dense plaques of striated and smooth muscle. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes).

Applications:
Suitable for use in Western Blot and Immunohistochemistry. Optimal dilutions to be determined by the researcher.

Recommended Dilution:
Western Blot: 1:16,000 (ECL)
Immunohistochemistry (frozen and paraffin sections, enhanced after microwave treatment): 1:500: Incubate for 1 hr at RT; extended with paraffin sections.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanGuinea pig
Concentration:
Not Determined
Immunogen:
C-terminal “C” domain of human recombinant plectin (aa 4367 - 4684).
Purity:
Serum
Form
Supplied as a liquid containing 0.09% sodium azide.
Specificity:
Recognizes human protein plectin at 53kD localizing to plasma membrane attachment sites of intermediate filaments and microfilaments (actin) such as desmosomes and hemi- desmosomes, Z-line structures and dense plaques of striated and smooth muscle. Specifically recognizes human glia cells. Strong positive reaction is also found with endothelia, fibroblasts and infiltrating cells (e.g. lymphocytes). Species Crossreactivity: bovine, mouse, Xenopus laevis, and fish. Also reactive with HL-60, HUVEC (human umbilical cord endothelial cell line), BMGE, and 3T3 cells.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Liu, C.G., et al. Human plectin: organization of the gene, sequence analysis, and chromosome localization (8q24). Proc Natl Acad Sci U S A. 93: 4278–83 (1996). 2. Wiche, G. Role of plectin in cytoskeleton organization and dynamics. J. Cell. Sci. 111: 2477–2486 (1998). 3. Ruhrberg, C. and F.M. Watt. The plakin family: versatile organizers of cytoskeletal architecture. Curr. Opin. Genet. Dev. 7: 392–397 (1997). 4. Foisner, R., et al. Protein kinase A and protein kinase C-regulated interaction of plectin with lamin B and vimentin. Proc. Natl. Acad. Sci. USA 88: 3812-3816 (1991)