Technical Data
P4230-13
Plakophilin 3
Description:
Several major families of cell adhesion molecules can be distinguished. These include the cadherins and N-CAMs, which play a crucial role in cell recognition and adhesion, while integrins are implicated in the adherence of cells to the extracellular matrix. Cadherins constitute a family of proteins, the members of which are differentially expressed in the different tissues. One of the best characterized members is E-cadherin, which is prevalent in epithelial tissues. It has been shown to play a crucial role in the process of tumor cell metastasis. NCAMs (neural cell adhesion molecules) exist in at least three forms of plasma membrane plycoproteins, which are expressed in a variety of tissues including most nerve cells. The integrins, finally, form a large family of glycosylated transmembrane proteins that act as dimers of an a- and a beta-subunit in interconnecting the cytoskeleton and the extracellular matrix.

Applications:
Suitable for use in Immunohistochemistry, Immunocytochemistry, Immunoprecipitation and Western Blot. Other applications not tested.

Recommended Dilution:
Immunocytochemistry: Methanol and paraformaldehyde-fixed cells.
Immunohistochemistry (Frozen and paraffn embedded): 1:25-1:200 (ABC)
Western Blot: 1:100-1:1000
Note: Pretreatment with 0.2% Triton-X 100 for 5 minutes for fixed cells and 15 minutes for frozen tissues, prior to incubation with primary antibody.
Optimal dilutions to be determined by researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b4A82Affinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanMouse
Concentration:
1mg/ml
Immunogen:
Synthetic peptide corresponding to aa570-589, CFTPQSRRLRELPLAADALTF of human plakophilin 3 (KLH via an additional cysteine at the N-terminus).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.2, 0.09% sodium azide. No stabilizing proteins added.
Specificity:
Recognizes human Plakophilin 3. Species Crossreactivity: mouse and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Bonne, S. et al., J. Cell Sci 112:2265-2276. (1999).