Plasminogen Activator Inhibitor 1 (PAI-1) is a glycoprotein found in plasma that is implicated in the processes of angiogenesis, hemostasis, tumor metastasis, fibrionlysis, ovulation, embryonic development and inflammation. The elevation of PAI-1 has shown to correlate with the prognosis of many cancers including breast cancer. PAI-1 is activated by the glycoprotein vitronectin. Vitronectin binds to PAI-1 and modulates fibrinolysis and cell migration. This binding has proven to be an important inhibitor of the activated form of protein C (APC).
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applilcations have not been tested.
Optimal dilutions to be determined by the researcher.
Non-glycosylated PAI-1: 43kD
Glycosylated PAI-1: 50kD
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Debrock, S., Declerck, P.J., Neutralization of plasminogen activator inhibitor-1 inhibitory properties: Identification of two distinct mechanisms. Biochim Biophys Acta 1337: 257-266 (1997). 2. Debrock, S., Declerck, P.J., Identification of a functional epitope in plasminogen activator inhibitor-1, not localized in the reactive center loop. Thromb. Haemostas. 79: 597-601 (1998). 3. Bijnens, A.P., Gils, A., Knockaert, I., Stassen, J.M., Declerck, P.J., Importance of the hinge region between alpha-helix F and the main part of serpins, based upon identification of the epitope of plasminogen activator inhibitor type 1 neutralizing antibodies. J. Biol. Chem. 275(9): 6375-80 (2000, Mar 3). 4. Ngo, T.H., Hoylaerts, M.F., Knockaert, I., Brouwers, E., Declerck, P.J., Identification of a target-site in plasminogen activator inhibitor-1 that allows neutralization of its inhibitory properties concomitant with an allosteric upregulation of its anti-adhesive properties. J. Biol. Chem. (2001, May 7). |
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