Technical Data
P4258-01
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
10ug
Growth Factors, Cytokines Storage: -20CShipping: RT
PDGFs are disulfide-linked dimers consisting of two 12.0-13.5kD polypeptide chains, designated PDGF-A and PDGF-B chains. The three naturally occurring PDGFs. (PDGF-AA, PDGF-BB and PDGF-AB) are potent mitogens for a variety of cell types including smooth muscle cells, connective tissue cells, bone and cartilage cells, and some blood cells. The PDGFs are stored in platelet alpha-granules and are released upon platelet activation. The PDGFs are involved in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubule epithelial cell development. Two distinct signaling receptors used by PDGFs have been identified and named PDGFR-alpha and PDGFR-beta. PDGFR-alpha is high-affinity receptor for each of the three PDGF forms. On the other hand, PDGFR-beta interacts with only PDGF-BB and PDGF-AB. PDGF is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TNF-alpha, TGF-beta and EGF.

Biological Activity: 10ng/ml of this lot of PDGF AA tested in an assay using 3T3/NIH broblasts supports a 2.3-fold increase in cell growth over control cultures without PDGF AA. The optimal PDGF concentration in cell culture can vary depending on cell type.

Sterility: Sterilized through a 0.2um membrane lter and packaged aseptically.

Rehydration: Use 10mM acetic acid or 4mM HCl containing 12mg BSA/ml for maximum recovery.

Notes: 1. In dilute solution, recombinant PDGF adheres tenaciously to surfaces, particularly glass; therefore use polypropylene containers, pipettes and tips. The use of carrier protein is necessary for dilution. 2. Biological activity is destroyed upon reduction.

Storage and Stability: Lyophilized: Stable 2 years at or below 4C from date of shipment. Rehydrated: 3 months at -20C. Aliquot to avoid repeated freezing and thawing.

Also Available:
P4258: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-01A: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
P4258-03: Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) Mab Mo xHu
P4258-04: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-04A: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-14: Platelet Derived Growth Factor AA (PDGF-AA) (Biotin) Pab Rb xHu
P4258-16A: Platelet Derived Growth Factor AA (PDGF-AA) Pab Rb xHu
P4258-19: Platelet Derived Growth Factor AA,AB,BB Neutralizing (PDGF-AA,AB,BB) Pab Gt xHu
Source: E. coli
Purity: Puri ed by HPLC. Determined by SDS-PAGE analysis: Reduced PDGF AA runs as a double band at 1418kD.
Form: Lyophilized from ~80ul of 0.01M acetic acid. Quantitated by amino acid analysis. Lyophilized powder.

Important Note: This product is intended for research use only, not for use in human, therapeutic or diagnostic applications.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.