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Technical Data |
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P4258-01B |
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: RT |
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Source: Recombinant corresponding to human Platelet Derived Growth Factor AA expressed in E. coli. Molecular Weight: ~28.5kD Endotoxin: ~0.1ng/ug Biological Activity: ED50 range=1-3ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application. Platelet derived growth factor is a mitogen for a variety of cell types, including connective tissue, smooth muscle tissue and fibroblasts. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% BSA, aliquot and store at-20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: 28.5kD |
Source: E. coli Purity: ~98% (SDS-PAGE, HPLC) Concentration: Not determined Form: Supplied as a lyophilized powder, carrier free. Sterile filtered prior to lyophilization. Reconstitute in sterile ddH2O, 0.1% BSA to 0.1-1mg/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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