Technical Data
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
Molecular Biology Storage: -20CShipping: RT
Recombinant corresponding to human Platelet Derived Growth Factor AA expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 range=1-3ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application. Platelet derived growth factor is a mitogen for a variety of cell types, including connective tissue, smooth muscle tissue and fibroblasts.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile ddH2O, 0.1% BSA, aliquot and store at-20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~98% (SDS-PAGE, HPLC)
Concentration: Not determined
Form: Supplied as a lyophilized powder, carrier free. Sterile filtered prior to lyophilization. Reconstitute in sterile ddH2O, 0.1% BSA to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.