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Technical Data |
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P4258-01E |
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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Recombinant human platelet derived growth factor AA is a homodimeric, non-glycosylated, polypeptide chain containing 2x125aa and having a total molecular weight of 28.5kD. The sequence of the first five N-terminal amino acids was determined to be Ser-Ile-Glu-Glu-Ala. Contains less than 1% dimers and aggregates. Source: E. coli Molecular Weight: ~28.5kD Biological Activity: The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is <1ng/ml, corresponding to a specific activity of 1MIU/mg. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: E. coli Purity: 97% (HPLC, FPLC and reducing and non-reducing SDS-PAGE). Form: Supplied as a lyophilized powder. Reconstitute using sterile deionized water to a concentration 100ug/ml. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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