Technical Data
P4258-01E
Platelet Derived Growth Factor AA, Recombinant, Human (PDGF-AA)
10ug
Molecular Biology Storage: -20CShipping: Blue Ice
Recombinant human platelet derived growth factor AA is a homodimeric, non-glycosylated, polypeptide chain containing 2x125aa and having a total molecular weight of 28.5kD. The sequence of the first five N-terminal amino acids was determined to be Ser-Ile-Glu-Glu-Ala. Contains less than 1% dimers and aggregates.

Source:
E. coli

Molecular Weight:
~28.5kD

Biological Activity:
The ED50 as determined by the dose-dependent proliferation of murine BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is <1ng/ml, corresponding to a specific activity of 1MIU/mg.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute with sterile buffer or ddH2O. Aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: 97% (HPLC, FPLC and reducing and non-reducing SDS-PAGE).
Form: Supplied as a lyophilized powder. Reconstitute using sterile deionized water to a concentration 100ug/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.