Technical Data
P4258-04F
PDGF-A, Recombinant, Human, Western Blot Control (Platelet-derived Growth Factor Subunit A, PDGF Subunit A, PDGF-1, Platelet-derived Growth Factor A Chain, Platelet-derived Growth Factor alpha Polypeptide, PDGFA, PDGF1)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
The term PDGF refers to a family of disulphide bondlinked dimeric isoforms that act as autocrine and paracrine growth factors and are produced by a variety of cell types other than platelets. They act as potent mitogens for almost all mesenchymally-derived cells. Aberrant expression is involved in certain cancers, fibroproliferative disorders and atherosclerosis. The protein also contributes to wound healing and neural regeneration. There are four members of the PDGF family PDGF A, PDGF B, PDGF C and PDGF D. Two distinct types of PDGF-A exist a short form that is soluble and a long form that is retained by the extracellular matrix..

Source:
Recombinant corresponding to aa87-196 from human PDGF-A, fused to 6x His-tag at N-terminal, expressed in E. coli.

Applications:
Suitable for use in Western Blot as a control. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: E. coli
Purity: Purified (SDS-PAGE).
Concentration: As reported
Form: Supplied as a liquid in 25mM Tris-HCl, pH6.8, 50mM DTT, 1% SDS, 0.1% Bromophenol Blue, 2.5% glycerol.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Raines, E.W. & Ross, R. (1982) J. Biol. Chem. 257(9): 5154-5160. 2. Bonthron, D.T. et al. (1988). Proc. Natl. Acad. Sci USA 85(5): 1492-1496.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.