Technical Data
P4258-10D
Platelet Derived Growth Factor AB, Recombinant, Human (PDGF-AB, PDGF alpha, beta Polypeptide, Becaplermin, Heterodimer Platelet-derived Growth Factor, Glioblastoma-derived T-cell Suppressor Factor, BSC-1 Cell Growth Inhibitor, Polyergin, Cetermin, TGFB2,
2ug
10ug
100ug
Molecular Biology Storage: -20CShipping: Blue Ice
PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TGF-alpha, TGF-beta and EGF.

Biological Activity:
Recombinant human PDGF-AB is fully biologically active when compared to standard. The ED50, determined by the dose-dependent proliferation of mouse 3T3 indicator cells is 1.4-2.1ng/ml

Amno Acid Sequence:
alpha chain MSIEEAVPAV CKTRTVIYEI PRSQVDPTSA NFLIWPPCVE VKRCTGCCNT SSVKCQPSRV HHRSVKVAKV EYVRKKPKLK EVQVRLEEHL ECACATTSLN PDYREEDTGR PRESGKKRKR KRLKPT
beta chain MSLGSLTIAE PAMIAECKTR TEVFEISRRL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRPTQVQLRP VQVRKIGIVR KKPIF

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months after receipt at -20C. Reconstitute with sterile ddH2O. Fo Aliquot to avoid repeated freezing and thawing. Store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
25.5kD
Source: E. coli
Purity: 95% as determined by reducing and non-reducing SDS-PAGE. Endotoxin: 0.1EU/ug as per LAL

Form: Supplied as a lyophilized powder from 10mM acetic acid.
Reconstitute with sterile ddH2O to a concentration of 0.1mg/ml. For long term storage, add a carrier protein (0.1% HSA or BSA).

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.