Technical Data
Platelet Derived Growth Factor AB, Recombinant, Human (PDGF-AB, PDGF alpha, beta Polypeptide, Becaplermin, Heterodimer Platelet-derived Growth Factor, Glioblastoma-derived T-cell Suppressor Factor, BSC-1 Cell Growth Inhibitor, Polyergin, Cetermin, TGFB2,
Molecular Biology Storage: -20CShipping: RT
Platelet-derived growth factor (PDGF) was discovered as a major mitogenic factor present in serum but absent from plasma. It was found to be secreted from the a-granules of platelets activated during the coagulation of blood to form serum. PDGF is a dimeric combination of two distinct but structurally related peptide chains designated A and B. The dimeric isoforms PDGF-AA, AB and BB are differentially expressed in various cell types and their effects are mediated through two distinct receptors, termed a and b. Differences exist in isoform binding to each receptor. In general, PDGF isoforms are potent mitogens for connective tissue cells, including dermal fibroblasts, glial cells, arterial smooth muscle cells and some epithelial and endothelial cells. In addition to its activity as a mitogen, PDGF is chemotactic for fibroblasts, smooth muscle cells, neutrophils and mononuclear cells. Other reported activities for PDGF include stimulation of granule release by neutrophils and monocytes, facilitation of steroid synthesis by Leydig cells, stimulation of neutrophil phagocytosis, inhibition of natural killer (NK) cell activity, stimulation of collagen synthesis, modulation of thrombospondin expression and secretion, stimulation of collagenase activity and secretion, induction of contraction of rat aorta strips in vitro, and transient induction of T cell IL-2 secretion accompanied by a down-regulation of IL-4 and IFN-y production, temporary effects that may allow clonal expansion of antigen-activated B and T helper lymphocytes prior to differentiation. PDGF also appears to be ubiquitous in neurons throughout the CNS, where it is suggested to play an important role in neuron survival and regeneration, and in mediation of glial cell proliferation and differentiation.

Recombinant mature human PDGF-A chain and PDGF-B chain expressed in E. coli and dimerized in vitro.

Biological Activity: Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected ED50 for this effect is 1-3ng/ml.

Sterility: Filtered through a 0.2um membrane and packaged aseptically. 0.1ng endotoxin per ug PDGF-AB as determined by the LAL method.

Reconstitution: Reconstitute with 4mM HCl or 10mM acetic acid containing 12mg/ml BSA is recommended.
Note: In dilute solution, PDGF adheres to surfaces, particularly glass. Therefore, use only polypropylene containers, pipettes and tips. Biological activity is destroyed upon reduction.

Storage and Stability: Stable for 2 years at 4C from date of shipment. Rehydrated: Stable for 3 months at -20C. Aliquot to avoid repeated freezing and thawing.
Source: Recombinant, Human from E. coli
Purity: SDS-PAGE Analysis: Reduced PDGF-AB runs at Mr 14kDa (B chain) and 17kDa (A chain). Nonreduced PDGF-AB runs at Mr ~28kDa. >97% pure.
Form: Lyophilized from 50ul of 10mM acetic acid.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.