Technical Data
Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)
Molecular Biology Storage: -20CShipping: RT
Platelet Derived Growth Factor (PDGF) is a potent mitogen for a wide range of cell types including fibroblasts, smooth muscle and connective tissue. PDGF, which is composed of a dimer of two chains termed the A chain and B chain, can be present as AA or BB homodimers or as an AB heterodimer.

Recombinant human PDGF-BB, 218 total amino acids, expressed in E. coli.

Molecular Weight: 24.3kD

Biological Activity:
The ED50 as determined by the dose-dependent stimulation of BALB/c 3T3 cells proliferation is < 1ng/ml

Endotoxin: 1EU/ug (LAL)

Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% (SDS-PAGE and HPLC)
Form: Supplied as a lyophilized powder.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Early rise in serum VEGF and PDGF levels predisposes patients with a normal MBL2 genotype to restenosis after eversion endarterectomy Stroke 38 (8), 2247-2253 (2007). 2. Sorafenib inhibits imatinib-resistant KIT and platelet-derived growth factor receptor beta gatekeeper mutants Clin. Cancer Res. 13 (11), 3363-3369 (2007).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.