Technical Data
Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)
Molecular Biology Storage: -20C/-70CShipping: Dry Ice
Recombinant corresponding to mouse Platelet Derived Growth Factor BB expressed in E. coli.

Molecular Weight:

Endotoxin: ~0.1ng/ug

Biological Activity:
ED50 range=1-5ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 100mM acetic acid, 0.1% BSA, aliquot and store at - 20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: ~95% (SDS-PAGE). Endotoxin: 0.1ng/ug.
Concentration: ~0.1mg/ml
Form: Supplied as a lyophilized powder, carrier free. Reconstitute in 100mM acetic acid, 0.1% BSA to 0.1-1mg/ml to regain full activity. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Bonthron, D.T., P. Sultan, and T. Collins (1991) Structure of the murine c-Sis protooncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor Genomics 10:287-2922. Kim, H.R., S. Upadhyay, S. Korsemeyer, and T.F. Deuel (1994) Platelet-derived growth factor (PDGF) B and A homodimers transform murine fibroblasts depending on the genetic background of the cell J. Biol. Chem. 269:30604-30608.3. Hoppe, J., V. Hoppe, T.A. Karenberg, A. Fenn, A. Simm, and A. Sachinidis (1994) Differential activation by platelet-derived growth factor-BB of mitogen activated protein kinases in starved or nonstarved AKR-2B fibroblasts. J. Cell. Physiol. 161:342-3504. Patel, B.K., L. Wang, C.C. Lee, W.G. Taylor, J.H. Pierce, and W.J. LaRochelle (1996) STAT6 and JAK1 are common elements in the platelet-derived growth factor and interleukin-4 signal transduction pathways in NIH 3T3 fibroblasts. J. Biol. Chem. 271:22175-221825. Chaudhary, L.R. and K.A. Hruska (2001) The cell survival signal Akt is differentially activated by PDGF-BB, EGF, and FGF-2 in osteoblastic cells. J. Cell Biochem.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.