Technical Data
P4258-15M
Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB)
10ug
|
Technical Data |
|
P4258-15M |
Platelet Derived Growth Factor BB, Recombinant, Mouse (PDGF-BB) |
10ug |
| Growth Factors, Cytokines | Storage: -20°C/-70°CShipping: Dry Ice |
|
Source: Recombinant corresponding to mouse Platelet Derived Growth Factor BB expressed in E. coli. Molecular Weight: ~25kD Endotoxin: ~0.1ng/ug Biological Activity: ED50 range=1-5ng/ml, determined by the dose-dependent proliferation of BALB/c 3T3 cells. The optimal concentration for each specific application should be determined by an initial dose-response assay. Storage and Stability: Lyophilized powder may be stored at 4°C for short-term only. Reconstitute to nominal volume by adding sterile 100mM acetic acid, 0.1% BSA, aliquot and store at - 20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Molecular Weight: ~25kD |
Source: E. coli Purity: ~95% (SDS-PAGE). Endotoxin: 0.1ng/ug. Concentration: ~0.1mg/ml Form: Supplied as a lyophilized powder, carrier free. Reconstitute in 100mM acetic acid, 0.1% BSA to 0.1-1mg/ml to regain full activity. Further dilutions should be made in low endotoxin medium or buffered solution with FBS or tissue culture grade BSA. Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
|
1. Bonthron, D.T., P. Sultan, and T. Collins (1991) Structure of the murine c-Sis protooncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor Genomics 10:287-2922. Kim, H.R., S. Upadhyay, S. Korsemeyer, and T.F. Deuel (1994) Platelet-derived growth factor (PDGF) B and A homodimers transform murine fibroblasts depending on the genetic background of the cell J. Biol. Chem. 269:30604-30608.3. Hoppe, J., V. Hoppe, T.A. Karenberg, A. Fenn, A. Simm, and A. Sachinidis (1994) Differential activation by platelet-derived growth factor-BB of mitogen activated protein kinases in starved or nonstarved AKR-2B fibroblasts. J. Cell. Physiol. 161:342-3504. Patel, B.K., L. Wang, C.C. Lee, W.G. Taylor, J.H. Pierce, and W.J. LaRochelle (1996) STAT6 and JAK1 are common elements in the platelet-derived growth factor and interleukin-4 signal transduction pathways in NIH 3T3 fibroblasts. J. Biol. Chem. 271:22175-221825. Chaudhary, L.R. and K.A. Hruska (2001) The cell survival signal Akt is differentially activated by PDGF-BB, EGF, and FGF-2 in osteoblastic cells. J. Cell Biochem.
|
||