Technical Data
P4258-15P
Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)
2ug
10ug
100ug
Molecular Biology Storage: -70CShipping: Blue Ice
PDGF is a mitogenic peptide growth hormone carried in the alpha-granules of platelets. It is released when platelets adhere to traumatized tissues. Connective tissue cells near the traumatized region respond by initiating the process of replication.The synthesis of PDGF can be induced by Interleukin-1, Interleukin-6, TNF-alpha, TGF-beta and EGF.

Endotoxin: <0.01ng/ug or <0.1EU/ug as per LAL

Biological Activity: PDGF-BB is fully biologically active when compared to standard. The ED50, calculated by the dose-dependent proliferation of BALB/c 3T3 indicator cells (measured by 3H-thymidine uptake) is < 1ng/ml, corresponding to a Specific Activity of 1x106IU/mg.

AA Sequence:
SLGSLTIAEP AMIAECKTRT EVFEISRRLI DRTNANFLVW PPCVEVQRCS GCCNNRNVQC RPTQVQLRPV QVRKIGIVRK KPIFKKATVT LGDHLACKCE TVAAARPVT

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O, aliquot and store at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
24.3 kD
Source: Recombinant, human, E. coli
Purity: 95% as determined by reducing and non-reducing SDS-PAGE.

Form: Supplied as a lyophilized powder. Reconstitute with sterile dH2O PBS to 0.1-1mg/ml, which can be further diluted into other aqueous solutions.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.