Technical Data
P4258-18A
Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent.

Source:
yeast

Biological Activity:
The material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells.

Storage and Stability:
Lyophilized powder may be stored at -20C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20C. Reconstituted product is stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Source: yeast
Purity: Purified. 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis.
Concentration: As reported
Form: Supplied as a lyophilized powder in PBS

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Ross, R. et al. (1986) Cell 46:155 2. Johnson, A. et al. (1984) EMBO J.3:921 3. Waterfield, M.D. et al. (1983) Nature 303: 35 4. Brake, A.J. et al. (1884) Proc. Natl. Acad. Sci. (USA) 81:4642

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.