Technical Data
P4258-18A
Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB)
10ug
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Technical Data |
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P4258-18A |
Platelet Derived Growth Factor BB, Recombinant, Human (PDGF-BB) |
10ug |
| Growth Factors, Cytokines | Storage: -20°CShipping: Blue Ice |
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This polypeptide is composed of two identical polypeptide chains attached by disulfide bonds. As it has been described in the natural product, PDGF-BB is partially clipped at Arg32-Thr33. This cleavage is visible upon reduction of the polypeptide with a reducing agent. Source: yeast Biological Activity: The material is fully active as determined by its mitogenic activity measured by stimulation of 3H-thymidine incorporation into human foreskin fibroblast cells. Storage and Stability: Lyophilized powder may be stored at -20°C. Reconstitute to with sterile dH2O or buffer, aliquot and store at -20°C. Reconstituted product is stable for 6 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. |
Source: yeast Purity: Purified. 97% pure by N-terminal aa sequencing, aa composition, HPLC analysis, and SDS gel electrophoresis. Concentration: As reported Form: Supplied as a lyophilized powder in PBS Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological. |
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1. Ross, R. et al. (1986) Cell 46:155 2. Johnson, A. et al. (1984) EMBO J.3:921 3. Waterfield, M.D. et al. (1983) Nature 303: 35 4. Brake, A.J. et al. (1884) Proc. Natl. Acad. Sci. (USA) 81:4642
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