Technical Data
P4258-18H
Platelet Derived Growth Factor CC, Recombinant, Human (PDGF-CC)
5ug
20ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
The platelet-derived growth factor (PDGF) family of heparin-binding growth factors consists of five known members, denoted PDGF-AA, PDGF-BB, PDGF-AB, PDGF-CC and PDGF-DD. The mature and active form of these proteins, an anti-parallel disulfide-linked dimer of two 12-14kD polypeptide chains, is obtained through proteolytic processing of biologically inactive precursor proteins, which contain an N-terminal CUB domain and a PDGF/VEGF homologous domain. The PDGFs interact with two related protein tyrosine kinase receptors, PDGFR- and PDGFR- , and are potent mitogens for a variety of cell types, including smooth muscle cells, connective tissue cells, bone and cartilage cells, and certain tumor cells. They play an important role in a number of biological processes, including hyperplasia, chemotaxis, embryonic neuron development, and respiratory tubules epithelial cell development. Mature PDGFs are stored in platelet -granules and are released upon platelet activation. PDGF-AA, -AB, -BB and CC signal primarily through the PDGF-R receptor, whereas PDGF-DD interacts almost exclusively with the PDGF-R receptor. Recombinant human PDGF-CC is a 25kD protein consisting of two identical disulfide-linked 114 amino-acid polypeptide chains.

Amino Acid Sequence:
MVVDLNLLTE EVRLYSCTPR NFSVSIREEL KRTDTIFWPG CLLVKRCGGN CACCLHNCNE CQCVPSKVTK KYHEVLQLRP KTGVRGLHKS LTDVALEHHE ECDCVCRGST GG

Biological Activity:
Determined by the dose-dependent stimulation of the proliferation of Balb/c 3T3 cells. The expected The ED50 for this effect is 15-20ng/ml.

Storage and Stability:
Lyophilized powder stored at -20C is stable for 12 months. Reconstituted product is stable for 3 months when stored at -20C in working aliquots with a carrier protein. Avoid freeze-thaw cycles. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Country of Origin:
USA
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1 ng/ug
Concentration: As reported
Form: Supplied as a lyophilized powder. Reconstitute with dH2O to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.