Technical Data
Platelet Factor 4 (PF4) Recombinant, Human
Molecular Biology Storage: -20CShipping: RT
Human Platelet Factor-4 (PF-4) is a 7.8kD protein containing 70aa which is structurally related to human IL-8 and GRO- . PF-4 is a chemoattractant for human fibroblasts and certain other cells.

Biological Activity:
The maximal chemotactic activity of human PF-4 on human fibroblasts as assayed in a modified Boyden chamber was achieved at a concentration range of 1.0-10.0ng/ml.

Responding Cells (partial list): Endothelial cells, Fibroblasts

Concentration Range for most in vitro applications: 1.0-50ng/ml

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with ddH2O, protein carrier. Aliquot and store at -20C. Reconstituted product is stable for 3 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
Source: E. coli
Purity: 98% by SDS-PAGE and HPLC analyses. Endotoxin: 0.1ng/ug
Form: Supplied as a lyophilized protein without additives.
Reconstitute with sterile ddH2O, protein carrier to 0.1-1mg/ml.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.