Technical Data
P4268
Plastin L (PLSL, L Plastin, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2)
Description:
L-plastin is a leukocyte specific actin bundling protein from fimbrin family of proteins. It has two actin-binding domains and two EF hand type calcium-binding domains. It gets phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosteronse in andrgen receptor positive prostrate and breast cancer cells through three androgen receptor binding elements located in the upstream region of the gene.

Applications:
Suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation. Other applications not tested.

Recommended Dilutions:
Western Blot: 1-2ug/ml for 2 hours at RT.
Immunofluorescence: Fixed in methanol or 3% paraformaldehyde.
Immunoprecipitation (Native): 2ug/mg protein lysate using Protein G.
Optimal dilution to be determined by researcher.

Positive Control:
Human polymorphonuclear neutrophils and monocytes, MCF-7, Jurkat cells.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG13F316Affinity Purified
SizeStorageShippingSourceHost
500ul-20CBlue IceHumanMouse
Concentration:
~0.2mg/ml
Immunogen:
Human recombinant L-plastin protein. Cellular Localization: Cytoplasmic. MW of Antigen: 62kD. Epitope: Headpiece region.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See P4268X.
Specificity:
Recognizes human LPL in Ca2+ independent fashion. Does not crossreact with T-or I-plastin. Does not cross react with mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Jones, S.L., Brown, E.J., J. Biol. Chem. 271: 14623-14630 (1996). 2. Lollike, K., et al., J. Biol. Chem. 276: 17762-69 (2001).