Technical Data
P4268-02
Plastin L (PLSL, L-Plastin, LC64P, Lymphocyte Cytosolic Protein 1, LCP1, LCP-1, Plastin 2, Plastin-2)
Description:
L-plastin is a leukocyte specific actin bundling protein from fimbrin family of proteins. It has two actin-binding domains and two EF hand type calciumbinding domains. It is phosphorylated on the serine residue in its headpiece region in response to a variety of inflammatory stimuli. It is hypothesized that L-plastin may play a role in regulating integrin mediated adhesion in leukocytes. L-plastin is expressed in majority of human cancer cell lines derived from solid tumors. Its expression is positively upregulated by testosteronse in androgen receptor positive prostrate and breast cancer cells, through three androgen receptor binding elements, located in the upstream region of the gene.

Applications:
Suitable for use in Immunoprecipitation, Western Blot and Immunofluorescence. Other applications not tested.

Recommended Dilution:
Western Blot: 1-2ug/ml for 2h, RT
Immunoprecipitation: 2ug/mg protein lysate
Optimal dilutions to be determined by the researcher.

Positive Control:
Human polymorphonuclear neutrophils, monocytes, MCF-7, Jurkat cells

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1SPM414Affinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Recombinant coresponding to L-plastin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.05% sodium azide.
Specificity:
Recognizes human L-plastin. Does not react with rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.