Technical Data
P4271-35
Plexin C1 (CD232, Virus-Encoded Semaphorin Protein Receptor, VESPR)
Description:
Plexin C1, also known as Virus-encoded semaphorin protein receptor (VESPR) and CD232, is a 210kD type I transmembrane glycoprotein in the C subfamily of the large plexin family. It is widely expressed in neuronal and non-neuronal fetal and adult tissues. Plexin C1 is a receptor for the semaphorin homolog A39R encoded within poxvirus genomes, as well as the herpes virus Sema protein. The large Plexin C1 extracellular domain (ECD) contains one Sema-domain and two cysteine-rich Met-related sequences (MRS). In the region used as immunogen, human Plexin C1 shares 92% amino acid sequence homology with mouse Plexin C1.

Applications:
Suitable for use in Western Blot, Flow Cytometry and Direct ELISA. Other applications not tested.

Recommended Dilution:
Western Blot: 0.1-0.2ug/ml; The detection limit is ~1ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 50ug/ml; Add 10ul of the diluted solution to 1-2.5x10e5 cells in a total reaction volume not exceeding 200ul. The binding of unlabeled antibodies may be visualized by adding 10ul of a 25ug/ml stock solution of a secondary developing reagent such as anti-goat lgG conjugated to a fluorochrome. Tested on white blood monocytes.
Direct ELISA: 0.5-1.0ug/ml; The detection limit is ~0.5ng/well.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanGoat
Concentration:
~0.2mg/ml
Immunogen:
E. coli-derived recombinant human Plexin C1 (aa49-161), Accession #: O60486. Species Sequence Homology: mouse (92%).
Purity:
Purified by Immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes human Plexin C1. In direct ELISAs and Western Blots, this antibody shows ~10% crossreactivity with recombinant mouse Plexin A1, A2, and A3, recombinant human Plexin B1, and <1% crossreactivity with recombinant human Plexin D1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.