Technical Data
P4271-45A
Plexin D1 (Biotin)
Description:
Plexin D1 is a type I transmembrane protein that is the prototype of the Plexin D subfamily in the Plexin family. It is expressed on multiple tissues including fetal neuronal and endothelial cells. Plexin D1 expression is also induced on tumor vasculature and tumor cells. The 1879 amino acid (aa) mature human Plexin D1 contains a large (1225 aa) extracellular domain with a 500 aa sema domain and three short MRS (Met-related sequences) cysteine-rich motifs. By alternative splicing, multiple Plexin-D1 isoforms may exist. Plexin D1 binds Sema4A to suppress angiogenesis. It also binds Sema3E directly to mediate repulsive vascular vessal guidance. In mouse, this interaction has been shown to be independent of Neuropilin and Sema3A. Over the extracellular region used as immunogen, human Plexin D1 shares 89% and 91% aa identity with the mouse and canine protein, respectively.

Applications:
Suitable for use in Western Blot and Flow cytometry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 0.1-0.2ug/ml. The detection limit is~1ng/lane under non-reducing and reducing conditions.
Flow Cytometry: 25ug/ml. Add 10ul of the diluted solution to 1-2.5 x 10e5 cells in a total reaction volume not exceeding 200ul. The binding of biotinylated antibodies may be visualized by adding a secondary developing reagent such as streptavidin conjugated to a fluorochrome.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding 1ml TBS pH7.3, 0.1% BSA. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceHumanGoat
Concentration:
~0.05mg/ml (after reconstitution)
Immunogen:
Recombinant corresponding to (aa47-1271 of human Plexin D1 extracellular domain expressed in NOS cells (Q9Y4D7). Species sequence homology: Mouse 89% and canine 91%.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS with BSA. Labeled with Biotin. Reconstitute with 1ml TBS, pH7.3, 0.1% BSA.
Specificity:
Recognizes human Plexin D1 extracellular domain. Crossreactivity: rmPlexin A1, rmPlexin A3, and rhPlexin B1 <1%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.