Technical Data
P4350-11
PNP (Purine Nucleoside Phosphorylase, Inosine Phosphorylase, NP)
Description:
Purine Nucleoside Phosphorylase (PNP) catalyzes the phophorolysis of N-ribosidic bonds of purine nucleosides and deoxynucleosides. Physiological substrates of PNP include inosine, guanosine, and 2'-deoxyguanosine, but not adenosine. PNP is expressed in most tissues, with markedly greater expression in lymphoid tissues. Genetic deficiencies of PNP result in severely compromised T-lymphocyte function and neurologic dysfunction. PNP is used in assays for the measurement of inorganic phosphate.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanSheep
Concentration:
~0.1mg/ml after reconstitution
Immunogen:
Recombinant corresponding to aa1-289 from human PNP expressed in E.coli.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder in PBS, trehalose. Reconstitute with sterile PBS to 0.2mg/ml.
Specificity:
Recognizes human PNP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Schramm, V.L. (1998) Annu. Rev. Biochem. 67:693. 2. Stoop, W. et al. (1977) N. Eng. J. Med. 296:651. 3. Markert, M.L. (1991) Immunodefic. Rev. 3:45. 4. Webb, M.R. (1992) Proc. Natl. Acad. Sci. USA. 89:4884.