		Technical Data
P4400-15C	Polymyxin B

Description:
The peptide antibiotic Polymyxin B (PMB) binds to bacterial endotoxin (lipopolysaccharide, LPS). The interaction of PMB with LPS involves ionic forces between amino groups in PMB and negatively charged phosphate and carboxyl groups in the lipid A-Kdo region. PMB has relevance for endotoxin research in at least two ways: first, PMB reacts with LPS of many species regardless of varied serospecificity, and thus it can be used as a general probe for measuring or detecting LPS or lipid A. Second, binding of PMB to LPS may result in neutralization of the detrimental effects of LPS either in vitro or in vivo. Monoclonal antibody enables the possibilities to study quantitatively the interaction of PMB and LPS.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:10
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgM	9G252	Supernatant

Size		Storage	Shipping	Source	Host
1ml		-20°C	Blue Ice		Mouse

Concentration:

Not determined.

Immunogen:

Whole bacteria of the Re chemotype of S. minnesota

Purity:

Supernatant

Form

Supplied as a liquid, 0.02% sodium azide.

Specificity:

Recognizes Polymyxin B. Binds to free Polymyxin B as well as to Polymyxin B already bound to LPS.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Appelmelk, B et al; Polymyxin B-horseradish pero1992, 207: 311