Polynuclear aromatic hydrocarbons or Polycyclic Aromatic Hydrocarbons (PAHs) are contain multiple benzene rings. They are generally found as components of fuels, oils and greases.
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.
Immunofluorescence (IC): 1:10-1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Benzo[a]pyrenyl-1-butyric acid conjugated to BSA. |
|Purified by affinity chromatography. >95% (SDS-PAGE)|
|Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.|
|Recognizes Polynuclear Aromatic Hydrocarbons. Inhibits DNA adduct formation (investigated in rat liver microsomes spiked with calf thymus DNA and 7,8-diol-B[a]P). Reactivity: Benzo[a]pyrene:100% Naphthalene:<1% Acenaphthene: 1%±0.2 Acenaphthylene: <1%Fluorene:5%±1 Phenanthrene:23%±4 Anthracene:25%±4 Fluoranthene:60% ±9 Pyrene:47%±7 Benzo[a]anthracene:44%±7 Chrysene:42%±6 Benzo[b]fluoranthene:26%±4 Benzo[k]fluoranthene:15%±2 Dibenzo[a,h]anthracene:9%±1 Benzo[g,h,i]perylene:15%±2 Indeno[1,2,3-c,d]pyrene:63%±10 Benzo[e]pyrene:55%±9 Perylene:8%±1|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Scharnweber T et al.. Fresenius J Anal Chem 371:578-85 (2001). 2. Suchanek M et al. Folia Biol (Praha) 47:106-7 (2001). 3 De Buck SS, et al., Carcinogenesis. 2005 Apr;26(4):835-44.|