Technical Data
P4415
Polynuclear Aromatic Hydrocarbons (PAH, Polycyclic Aromatic Hydrocarbons)
Description:
Polynuclear aromatic hydrocarbons or Polycyclic Aromatic Hydrocarbons (PAHs) are contain multiple benzene rings. They are generally found as components of fuels, oils and greases.

Applications:
Suitable for use in ELISA and Immunofluorescence. Other applications not tested.

Recommended Dilutions:
ELISA: 1:2000
Immunofluorescence (IC): 1:10-1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG14i314Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~1mg/ml
Immunogen:
Benzo[a]pyrenyl-1-butyric acid conjugated to BSA.
Purity:
Purified by affinity chromatography. >95% (SDS-PAGE)
Form
Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide.
Specificity:
Recognizes Polynuclear Aromatic Hydrocarbons. Inhibits DNA adduct formation (investigated in rat liver microsomes spiked with calf thymus DNA and 7,8-diol-B[a]P). Reactivity: Benzo[a]pyrene:100% Naphthalene:<1% Acenaphthene: 1%0.2 Acenaphthylene: <1%Fluorene:5%1 Phenanthrene:23%4 Anthracene:25%4 Fluoranthene:60% 9 Pyrene:47%7 Benzo[a]anthracene:44%7 Chrysene:42%6 Benzo[b]fluoranthene:26%4 Benzo[k]fluoranthene:15%2 Dibenzo[a,h]anthracene:9%1 Benzo[g,h,i]perylene:15%2 Indeno[1,2,3-c,d]pyrene:63%10 Benzo[e]pyrene:55%9 Perylene:8%1
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Scharnweber T et al.. Fresenius J Anal Chem 371:578-85 (2001). 2. Suchanek M et al. Folia Biol (Praha) 47:106-7 (2001). 3 De Buck SS, et al., Carcinogenesis. 2005 Apr;26(4):835-44.