Description:
Polynuclear aromatic hydrocarbons or Polycyclic Aromatic Hydrocarbons (PAHs) are contain multiple benzene rings. They are generally found as components of fuels, oils and greases.
Applications:
Suitable for use in ELISA and Immunocytochemistry. Other applications not tested.
Recommended Dilutions:
ELISA: 1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
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| Type | Isotype | Clone | Grade |
|---|
| Mab | IgG1 | 4i314 | Affinity Purified |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ug | | -20°C | Blue Ice | | Mouse |
|
| Concentration: |
| ~1mg/ml |
| Immunogen: |
| Benzo[a]pyrenyl-1-butyric acid conjugated to BSA. |
| Purity: |
| Purified by affinity chromatography. >95% (SDS-PAGE) |
| Form |
| Supplied as a liquid in PBS, pH 7.4, 15mM sodium azide. |
| Specificity: |
| Recognizes Polynuclear Aromatic Hydrocarbons. Inhibits DNA adduct formation (investigated in rat liver microsomes spiked with calf thymus DNA and 7,8-diol-B[a]P). Reactivity: Benzo[a]pyrene:100% Naphthalene:<1% Acenaphthene: 1%±0.2 Acenaphthylene: <1%Fluorene:5%±1 Phenanthrene:23%±4 Anthracene:25%±4 Fluoranthene:60% ±9 Pyrene:47%±7 Benzo[a]anthracene:44%±7 Chrysene:42%±6 Benzo[b]fluoranthene:26%±4 Benzo[k]fluoranthene:15%±2 Dibenzo[a,h]anthracene:9%±1 Benzo[g,h,i]perylene:15%±2 Indeno[1,2,3-c,d]pyrene:63%±10 Benzo[e]pyrene:55%±9 Perylene:8%±1 |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
1. Scharnweber T et al.. Fresenius J Anal Chem 371:578-85 (2001). 2. Suchanek M et al. Folia Biol (Praha) 47:106-7 (2001). 3 De Buck SS, et al., Carcinogenesis. 2005 Apr;26(4):835-44.
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