Technical Data
P4444-26
Polyethylene Glycol (PEG) (Biotin)
Description:
Polyethylene glycol (PEG) is a family of long chain polymers attached to a glycerine backbone. It is a nonionic, nontoxic, biocompatible, strongly hydrophilic polymer, which has a large exclusion volume in aqueous solution. The covalent attachment of PEG is now commonly used to modify a variety of proteins and drugs. The modification of a biopharmaceutical with polyethylene glycol (PEG) increases its hydrodynamic radius, reduces immunogenicity and proteolytic cleavage. Other benefits include decelerated renal excretion, improved stability towards proteolysis and increased solubility of the biopharmaceutical in aqueous solutions. As examples, PEG-adenosine deaminase (Adagen) is used for the treatment of severe combined immunodeficiency syndrome, PEG-asparaginase (Oncaspar) is used for the treatment acute lymphoblastic leukemia, PEG-interferon alpha 2a (Pegasys) is used for the treatment Hepatitis C, Branched PEG-anti-VEGF aptamer (Pegaptanib, Macugen) is used for the treatment Macular degeneration (age-related). An Anti-PEG antibody can be used to monitor a drugs pharmacokinetics, including distribution, metabolism and excretion.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry Other applications not tested.

Recommended Dilutions:
ELISA: 0.5ug/ml for use as a detection antibody. Use 5-10ug/ml to coat plates for use as capture antibody.
Western Blot: 15.6ng/ml (1:5000-1:20,000)
Immunohistochemistry: 1:100
Optimal dilutions to be determined by the researcher.

Affinity Constant:
1.58nM

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9E454Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceRabbit
Concentration:
~1mg/ml
Immunogen:
KLH-PEG with terminal methoxy group.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.05% BSA, 0.01% sodium azide, 40% glycerol. Labeled with Biotin.
Specificity:
Recognizes the terminal methoxy group of the PEG molecule. Recognizes various length Y-chain PEG molecules as well as single chain PEG molecules with equal affinity. Does not crossreact with non-specific targets in blood or serum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Guiotto, A., et al., Bioorg. Med. Chem. 12: 5031 (2004). 2. Wong, S.S., Reactive groups of proteins and their modifying agents. In Chemistry of Protein Conjugation and Cross-linking, p. 13, CRC Press (1991). 3. Caliceti, P., et al., J. Bioact. Comp. Polym. 8: 41 (1993). 4. Leenders, F., Biopharmaceuticals 6: 39-40 (2006). 5. Veronese, F.M. & Pasut, G., Drug Discov. Today 10(21): 1451-1458 (2005).