Technical Data
P4460
Polysialic Acid NCAM (PSA-NCAM)
Description:
Polysialic acid (PSA) is a long, linear homopolymer that is attached to the neural cell adhesion molecule (NCAM). It has the remarkable ability to down-regulate a wide variety of contact-dependent cell interactions. This activity is believed to stem from the ability of the charged polymer to occupy a large hydrated volume and thereby cause a direct physical hindrance of cell–cell contact. The regulated expression of PSA has been shown to promote a reduction in cell interactions that can create permissive conditions for changes in the structure of a variety of tissues.
The broadest expression of PSA occurs on precursor cells during early development and plays a role in promotion of the migration of these cells after cell division. In most cases, PSA expression is lost after the migration process is completed. However, the axons of neurons often retain PSA to bundle, sprout, and branch appropriately during axon path-finding.

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry and RIA. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:500-1:1000
Immunocytochemistry: 1:200-1:400; Works on live and fixed cells.
Immunohistochemistry: 1:200-1:400; Works on tissue sections either frozen or fixed with any kind of fixative.
Immunohistochemistry (Paraffin): 1:100. Requires retrieval with EDTA, pH 8.0. Note: Variable results have been obtained on paraffin sections.
Optimal dilutions to be determined by the researcher.

Recommended Control Tissue:
Young rat hippocampus, human hippocampus

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgM2Q130Ascites
SizeStorageShippingSourceHost
50ul-20°CBlue IceMouseMouse
Concentration:
Not Determined
Immunogen:
Viable Meningococcus group B (strain 355) (NM_000615.5).
Purity:
Ascites
Form
Supplied as a liquid in 50% glycerol. No preservatives added.
Specificity:
Recognizes mouse Polysialic Acid NCAM at 150-300kD. Works well on all vertebrate species expressing PSA-NCAM.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Koizumi, Hiroyuki, et al., Nat Neurosci. 9: 779-786 (2006). 2. Ramirez-Castillejo, C., et al., Nature Neurosci. 9: 331-339 (2006). 3. Ng, K.L., et al., Science 308: 1923-1927 (2005). 4. Scheffler, B., et al., Proc. Nat. Acad. Sci. USA 102(26): 9353-9358 (2005). 5. Medrano, S., et al., Neurobiol. Aging 30: 483-497 (2009).