Technical Data
Polyubiquitin, Lysine 48-Linkage Specific
Ubiquitin is a conserved polypeptide unit that plays an important role in the ubiquitin-proteasome pathway. Ubiquitin can be covalently linked to many cellular proteins by the ubiquitination process, which targets proteins for degradation by the 26S proteasome. Three components are involved in the target protein-ubiquitin conjugation process. Ubiquitin is first activated by forming a thiolester complex with the activation component E1; the activated ubiquitin is subsequently transfered to the ubiquitin-carrier protein E2, then from E2 to ubiquitin ligase E3 for final delivery to the epsilon-NH2 of the target protein lysine residue (1-3). The ubiquitin-proteasome pathway has been implicated in a wide range of normal biological processes and in disease-related abnormalities. Several proteins such as ikB, p53, cdc25a and bcl-2 have been shown to be targets for the ubiquitin-proteasome process as part of regulation of cell cycle progression, differentiation, cell stress response and apoptosis (4-7). Substrate proteins are linked to ubiquitin using seven distinct ubiquitin lysine residues (Lys6, Lys11, Lys27, Lys29, Lys33, Lys48 and Lys63). Formation of a polyubiquitin chain occurs when a lysine residue of ubiquitin is linked to the carboxy-terminal glycine of another ubiquitin. Proteins polyubiquinated at specific lysine residues display a tendency to be targeted for different processes; K48-linked polyubiquitin chains mainly target proteins for proteasomal degradation while K63-linked polyubiquitin regulates protein function, subcellular localization, or protein-protein interactions (8).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS,
0.1% Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceRabbit
Not determined
Synthetic peptide corresponding to the Lys48 branch of human diubiquitin chain (P62988).
Purified by Protein A affinity chromatography and peptide affinity chromatography.
Supplied as a liquid in 10mM HEPES, pH 7.5, 150mM sodium chloride, 0.1mg/ml BSA, 50% glycerol.
Recognizes polyubiquitin chains formed by Lys48 residue linkage. Species Crossreactivity: human, mouse, rat, hamster, monkey, mink, chicken, D. melanogaster, xenopus, zebrafish, bovine, canine, porcine, S. cerevisiae, C. elegans, equine. Slight cross-reactivity with linear polyubiquitin chain. No cross-reactivity with monoubiquitin or polyubiquitin chains formed by linkage to different lysine residues.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Ciechanover, A. (1998) EMBO J. 17, 7151-7160. (2) Hochstrasser, M. (2000) Nat. Cell Biol. 2, E153-E157. (3) Hochstrasser, M. (2000) Science 289, 563-564. (4) Bernardi, R. et al. (2000) Oncogene 19, 2447-2454. (5) Aberle, H. et al. (1997) EMBO J. 16, 3797-3804. (6) Salomoni, P. and Pandolfi, P.P. (2002) Nat. Cell Biol. 4, E152-E153. (7) Jesenberger, V. and Jentsch, S. (2002) Nat. Rev. Mol. Cell
Biol. 3, 112-121. (8) Komander, D. (2009) Biochem Soc Trans 37, 937-53.