Technical Data
P5600-08
PRAM1 (PML-RARA Target Gene Encoding Protein)
Description:
PRAM-1 is a 97kD adaptor protein, which is expressed and regulated during normal human myelopoiesis.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1-3ug/ml, Detects a band of approximately 90-100kD in Jurkat cell lysates. An overnight incubation time of 12 hours is recommended
Optimal working dilutions to be determined by researcher.

Recommended Secondary Reagents:
Donkey Anti Sheep/Goat IgG: HRP

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add glycerol (40-50%) and aliquot. Freeze at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Peptie sequence DFCDPLENQPLPLGR corresponding to the C-terminus of PRAM1 (NP_115528).
Purity:
Purified by affinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3 with 0.02% sodium azide, 0.5% BSA.
Specificity:
Recognizes human PML-RARA target gene encoding protein.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Moog-Lutz, C. et al. (2001). PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute pomyelocytic leukaemia cells. J. Biol. Chem. 276 (25) 22 375-81

PMID: 11 301322