PRAM-1 is a 97kD adaptor protein, which is expressed and regulated during normal human myelopoiesis.
Suitable for use in ELISA and Western Blot. Other applications not tested.
Western Blot: 1-3ug/ml, Jurkat cell lysates observed on ~90-100kD bands
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
|Synthetic peptide corresponding to DFCDPLENQPLPLGR, from human PRAM1, at C-terminal (NP_115528.3).|
|Purified by immunoaffinity chromatography.|
|Supplied as a liquid in Tris saline, 0.02% sodium azide, pH 7.3, 0.5% BSA.|
|Recognizes human PRAM1. Species sequence homology: porcine.|
|Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.|
1. Moog-Lutz C, Peterson EJ, Lutz PG, Eliason S, Cave-Riant F, Singer A, Di Gioia Y, Dmowski S, Kamens J, Cayre YE, Koretzky G. PRAM-1 is a novel adaptor protein regulated by retinoic acid (RA) and promyelocytic leukemia (PML)-RA receptor alpha in acute promyelocytic leukemia cells. J Biol Chem 2001 Jun 22;276(25):22375-81.|