Technical Data
P6300-81
Presenilin Enhancer Protein 2 (Pen2)
Description:
Pen-2 is an essential component of the gamma-secretase complex, which plays a central role in the pathogenesis of Alzheimer’s disease, and is also required for Notch signaling. The gamma-secretase complex is composed of a presenilin heterodimer, a highly glycosylated form of nicastrin, Aph-1 and Pen-2. The presenilin dimer contains the active site of the gamma-secretase complex, and the other three proteins act as necessary co-factors in the assembly of the active form of the gamma-secretase enzyme.
Aph-1 and Pen-2 were first identified in a C. elegans genetic screen as transmembrane proteins that are required for the activity and accumulation of gamma-secretase. Pen2 is critical for presenilin 1 endoproteolysis, while Aph-1 plays a regulatory role in the stabilization of presenilin 1.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Western Blot: 1-3ug/ml Identifies a band at ~14kD. Bands of unknown origin between ~30 and 100kD are also observed. Tested on human HEK293 cell lysates, Pen-2-HA-transfected cell lysates and mouse brain homogenates
Immunoprecipitation: 5-10ug/ml Tested on fetal mouse brain homogenates and HEK293 cell lysates.
Immunohistochemistry: 0.5-1ug/ml Tested on mouse brain tissue sections, fixed in 4% paraformaldehyde.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20°CBlue IceHumanRabbit
Concentration:
~0.25mg/ml
Immunogen:
Synthetic peptide derived from the N-terminal region of the human Presenilin Enhancer Protein 2.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human Presenilin Enhancer Protein 2, Mr ~14kD. Species Crossreactivity: Mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Steiner, H., et al., J. Biol. Chem. 277(42): 39,062-39,065 (2002). 2. Crystal, A.S., et al., J. Biol. Chem. 278(22): 20,117-20,123 (2003). 3. LaVoie, M.J., et al., J. Biol. Chem. 278(39): 37,213-37,222 (2003). 4. Francis, R., et al., Dev. Cell 3(1): 85-97 (2002). 5. Luo, W., et al., J. Biol. Chem. 278(10): 7850-7854 (2003).