Technical Data
ProCollagen Type I, NT
Collagen type I is present in soft connective tissue and bone where it represents approximately 90% of the organic matrix. Collagen type I is produced from procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. The propeptide levels can be measured in serum as indicators of collagen production. Elevated values of these propeptides in serum are found in patients with various bone metabolism defects. Procollagen type I C-terminal propeptide (PICP) has a molecular weight of approximately 100kD and the normal range in serum is 100-300ug/ml.

Suitable for use in Immunohistochemistry. Not recommended for Western Blot. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (Fresh frozen tissue sections): 1:10,000-1:50,000
Immunohistochemistry (FFPE): 1:100-1:1000; Pretreatment with 1% trypsin, 20 minutes at RT.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20°CBlue IceHumanRat
Not Determined
Human Pro-collagen I
Supplied as a liquid.
Recognizes human Pro-collagen I. Labels the amino-terminal pro-peptide. Localized intracellularly in cells producing Pro-collagen I. Does not stain mature collagen fibers in tissue.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Abdulghani, A.A., et al., Disease Management and Clinical Outcomes. 1(4): 136-141 (1998).