17 alpha Hydroxyprogesterone, Human, BioAssay™ ELISA Kit
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The steroid 17 alpha-OH Progesterone is produced by the adrenal cortex and gonads. The 17 alpha-OH Progesterone has little progestational activity, but it is of intense clinical interest because it is the immediate precursor to 11-desoxycortisol (Cpd-S). Cpd-S is produced by 21- hydroxylation of 17 OHP, measurement of 17aOHP is a useful indirect indicator of 21-hydroxlase activity. In congenital 21-hydroxylase deficiency, the most common variety of congenital adrenal hyperplasia (CAH), 17 a OHP is secreted in abundant excess. Measurement of 17 alpha-OHP is therefore valuable in the initial diagnosis of CAH.
Serum. Other fluids not optimized.
For quantitative determination of 17 alpha Hydroxyprogesterone in human serum.
0.11ng/ml; 75 min assay
5-30 day: <0.7-2.5
31-60 day male: 0.8-5.0
31-60 day female: 0.5-2.3
3-14 yrs: 0.07-1.7
Follicular phase: 0.2-1.3
Luteal phase: 1.0-4.5
The principle of the following enzyme immunoassay test follows the typical competitive binding scenario. Competition occurs between an unlabeled antigen (present in standards, controls and patient samples) and an enzyme-labelled antigen (conjugate) for a limited number of antibody binding sites on the microwell plate. The washing and decanting procedures remove unbound materials. After the washing step, the enzyme substrate is added. The enzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed is inversely proportional to the concentration of 17alpha-OHP in the sample. A set of standards is used to plot a standard curve from which the amount of 17alpha-OHP in patient samples and controls can be directly read
T9006-04T1: Microwell Strip Plate: 1x96 wells
T9006-04T2: Standard,0ng/ml: 1x2ml
T9006-04T3: Standard, 0.15ng/ml: 1x600ul
T9006-04T4: Standard, 0.5ng/ml: 1x600ul
T9006-04T5: Standard, 1.5ng/ml: 1x600ul
T9006-04T6: Standard, 3ng/ml: 1x600ul
T9006-04T7: Standard, 7.5ng/ml: 1x600ul
T9006-04T8: Standard, 20ng/ml: 1x600ul
T9006-04T9: Control 1: 1x600ul
T9006-04T10: Control 2: 1x600ul
T9006-04T11: 17-OHP (HRP), 100X: 1x300ul
T9006-04T12: Assay Buffer: 1x20ml
T9006-04T13: Wash Buffer, 10X: 1x50 ml
T9006-04T14: TMB Solution: 1x16ml
T9006-04T15: Stop Solution, H2SO4: 1x6ml
Storage and Stability:
Store all components at 4°C. Once standards and controls are opened, aliquot and store at -20°C. Stable for 6 months after receipt. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.