		Technical Data
P9007-15E1	Progesterone Receptor (PgR)

Description:
Human PgR exists in two forms: 116kD (B-form) and 81kD (A-form). It acts as ligand activated transcription factor to regulate expression of the target genes. Null mutation in PgR gene leads to pleiotrophic reproductive abnormalities.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin-fixed, paraffin-embedded): 1:50-1:100 for 20 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
T47D cells, Breast carcinoma

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Purified

Size		Storage	Shipping	Source	Host
250ul		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.2mg/ml

Immunogen:

Recombinant protein encoding human progesterone receptor aa412-526. Cellular Localization: Nuclear. MW of Antigen: 81kD and 116kD.

Purity:

Purified

Form

Supplied as a liquid in PBS, pH 7.6, 0.2% BSA, 15mM sodium azide.

Specificity:

Recognizes human Progesterone Receptor.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.