Technical Data
P9007-34C
Progranulin, Recombinant, Human (Granulins, GRN, Proepithelin, PEPI)
10ug
Growth Factors, Cytokines Storage: -20CShipping: Blue Ice
Progranulin, also called proepithelin and PC cell-derived growth factor, is a single precursor protein of granulins which are a family of secreted, glycosylated peptides that are cleaved from a single precursor protein with 7.5 repeats of a highly conserved 12-cysteine granulin/epithelin motif. Granulins are a variety of active, 6kD peptides and named granulin A (epithelin 1), granulin B (epithelin 2), granulin C, etc. Both the peptides and intact progranulin protein regulate cell growth. However, different members of the granulin protein family may act as inhibitors, stimulators, or have dual actions on cell growth. Granulin family members are important in normal development, wound healing, and tumorigenesis. Progranulin Human Recombinant fused to FLAG at C-terminus produced in HEK is a single, glycosylated, polypeptide chain containing 593aa and having a molecular mass of 88kD. The Progranulin is purified by standard chromatographic techniques.

Source:
Recombinant corresponding to human progranulin expressed in HEK293 cells.

Molecular Weight:
~88kD

Biological Activity:
1.The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in THP-1 cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37C 0, 30, 60, min, respectively by adding the recombinant protein (100ng/ml) to the THP-1 monocyte cell, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 1, 2, and 3 were subjected to THP-1 monocyte cell treatments over 0, 30, 60 mins, respectively.
2. The effects of phospho-ERK1/2 and non phospho-ERK1/2 by human progranulin-FLAG in MCF10A cells. To examine the signal of phospho-p44/42 MAPK and p44/42 MAP kinase, reactions were carried out at 37C over 0, 10, 30, 60 mins, respectively by adding the recombinant protein (500ng/ml) to the MCF10A human breast epithelial cells, which was maintained with serum starvation for 24hrs. Recombinant proteins in lanes 2, 3, and 4 were subjected to MCF10A human breast epithelial cell treatments over 0, 10, 30, 60 mins, respectively.

Endotoxin: <0.1ng/ug

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 6 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Molecular Weight:
~88kD
Source: HEK293 cells
Purity: ~90% (HPLC, SDS-PAGE)
Concentration: ~1mg/ml
Form: Supplied as a liquid in PBS.

Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.