		Technical Data
P9009-10AX	Prolactin (BSA & Azide Free) (LTH, Luteotropic Hormone, Lactogenic Hormone)

Description:
Prolactin is a pituitary hormone involved in the stimulation of milk production, salt and water regulation, growth, development and reproduction. Prolactin producing cells make up ~20% of the pituitary.

Applications:
Suitable for use in ELISA and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
ELISA: Capture or tracer
Immunohistochemistry (Formalin/paraffin): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
Pituitary gland

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	3F319	Affinity Purified

Size		Storage	Shipping	Source	Host
100ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Human prolactin. Cellular Localization: Cytoplasmic

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. Also available with BSA and azide. See P9009-10A.

Specificity:

Recognizes human prolactin at ~23.5kD.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Staindl B, et al. J. Endocrinology. 114: 311–318 (1987). 2. Nicoll C S, et al. Endocrine Reviews. 7:169–203 (1986). 3. Von Werder K. In: The Pituitary Gland: 405-439. Ed. Imura H. New York, Raven Press (1985).