Technical Data
P9009-53A
Prolactin Receptor (PRLR, PRL-R, PRL Receptor)
Description:
Prolactin (PRL) is a hormone involved in a variety of important functions including ion transport and osmoregulation, stimulation of milk, protein synthesis as well as the regulation of numerous reproductive functions. PRL exerts its influence on different cell types through a signal transduction pathway which begins with the binding of the hormone to a transmembrane PRL receptor. Immunoreactive PRL receptor, a member of the cytokine receptor family, varies in size (short and long forms) with tissue source and species, from ~40kD to 100kD. The PRL receptor consists of at least three separate domains: an extracellular region with 5 cysteines which contains the prolactin binding site, a single transmembrane domain and a cytoplasmic region, the length of which appears to influence ligand binding and regulate cellular function.

Applications:
Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml detected PRLR on NB2 cell lysates.
Immunohistochemistry (Paraffin): Staining of PRL receptor in NB2 cells yields a staining pattern consistent with cytoplasmic vesicular staining.
Immunocytochemistry: 5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a9A441Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Purified rat liver PRL receptor.
Purity:
Purified by affinity chromatography
Form
Supplied as a lyophilized powder. No preservative added. Reconstitute with 100ul sterile PBS.
Specificity:
Recognizes the rat Prolactin Receptor at ~42kD. Does not crossreact with the Growth Hormone receptor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dardenne M et al. Endocrinology 134 (5):2108-14 (1994). 2. Rui H et al. Endocrinology 135 (4):1299-306 (1994). 3. Gagnerault MC et al. J Immunol 150 (12):5673-81 (1993). 4. Dardenne M et al. Proc Natl Acad Sci USA 88:9700-4 (1991). 5. Rozakis-Adcock M., Kelly PA. J Biol Chem 266 (25):16472-7 (1991). 6. Okamura H et al. Endocrinology 124 (5):2499-508 (1989).